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An unbiased and complete record of how a cholesteric sample acts on the measuring radiation can be obtained by ellipsometry This method (compare Sec 64) yields a comprehensive description of the state of polarization including the degree of polarization (Riiseler, 1990) An adequate simulation can be based on the Berreman formalism (1972) rendering possible a study of particularities observed, such as pronounced depolarization related to the selective reflection band (Reins et al, 1994) Several models were proposed to explain the twisting influence of the chiral molecules (Goossens, 197 1 ; Stegemeyer and Finkelmann, 1973; Finkelmann and Stegemeyer, 1974) It might be not too surprising that the orientational order in the vicinity of a dissymmetric molecule experiences a small twist, however, the tendency of keeping neighboured molecules as parallel as possible, causes a long-range collective reorientation by which new macroscopic properties appear.

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The phenomenon of induced cholesteric solutions can be considered as an amplification of chirality by transforming it from the intramolecular level into macroscopic order Induced cholesteric solutions proved to be an often very sensitive tool to reveal and to characterize molecular chirality, the experimental data can be achieved from simple infrared spectra For a detailed interpretation of the cholesteric optical activity, the optical model used must account for the often masked anisotropy..

0 = i x i i = 1,

Figure 4.4 Even if no CT1 LSPs are established, the bandwidth allocated for CT1 cannot be used for CT0

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The first present-day infrared spectrometers used prism monochromators During the 1960's, these were replaced by grating instruments, which have a larger optical conductance throughout the entire spectral range In the seventies, interferometers were introduced, since they further increased the optical conductance considerably (Jacquinot, 1954), combined with the multiplex or Fellgett advantage (Fellgett, 1958) (see Sec 334) Raman spectroscopy underwent almost the same change in instrumentation from prism to grating spectrometers However, interferometers were only seldom used (Jennings, Weber and Brault, 1986) The Raman spectrum to be analyzed contains mainly the exciting radiation with the frequency 3 0 , which is a result of Rayleigh and Tyndall scattering as well as reflection and refraction at particles and cuvettes.

Since long-chain fatty acids are poorly soluble in aqueous media, they must be transported in the plasma complexed with albumin. When a fatty acid dissociates from albumin it is transferred from the capillary lumen through the capillary endothelium and interstitial space to the cells below. Long-chain (ClGC20) fatty acids enter cells both by simple diffusion and by carrier-mediated transport. The diffusion mechanism involves initial penetration of the outer leaflet of the plasma membrane by the hydrophobic tail of the fatty acid, followed by a flip-flop within the membrane. Thus, when the fatty acid emerges on the cytosolic side of the membrane, the carboxyl group of the fatty acid enters the cytosol ahead of the hydrocarbon tail. There are three major fatty acid transporter proteins in the plasma membranes of human cells: fatty acid transporter (FAT/CD36), plasma membrane fatty acid

This radiation is accompanied by very strong lines and 'wings', which are due to rotational and translational vibrations of molecules in the condensed phase, with wavenumber shifts of up to about 80 cm-' from the exciting line The 'real' Raman lines have an intensity which is lower by several orders of magnitude, 10-' .. 10 1 2 The statistical noise of the quantum flux of the exciting radiation and the 'Rayleigh wings', ie, the shot noise, is according to Poisson statistics proportional to the square root of the intensity When the interferogram is transformed into a spectrum, this noise is distributed and appears as a background noise throughout the entire spectrum It is even stronger than the strongest Raman lines This multiplex disadvantage (Hirschfeld, 1976 b) has prevented the use of interferometers for Raman spectroscopy.

However, if the Rayleigh line and its wings are completely eliminated from the radiation to be analyzed, then Raman spectra can be recorded In this case, the Jacquinot advantage is complemented by a multiplex advantage (Secs 352, 355) As early as 1954, Jacquinot pointed out that interferometers have a considerably higher optical conductance than prism or grating spectrometers In order to quantify this relation we use Eqs 31-33 and 31-39 and add G or I as a superscript to designate the spectral optical conductance of grating instruments and interferometers, respectively: (31-33 a).

objects that can t be reached by any path starting from a root. Roots are the starting points for the garbage collector, fixed places that are always guaranteed to exist. In Java, the roots include static fields in classes and locals on the stack. This is shown in Figure 28-5. Anything that the garbage collector can t reach from one of the program s roots by any path is considered garbage.

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